When it comes to identifying proteins in research or developing therapies for clinical use, antibodies are clearly the most suitable option. Since Georges J. F. Kohler and César Milstein first described hybridoma technology for the production of monoclonal antibodies in 19741, antibodies have become indispensable for molecular biology and can be produced in a wide variety of hosts.
However, since then the original technique has been modified with variants that meet many special requirements3. With so many options available, how do you decide whether monoclonal or polyclonal antibodies are right for your experiment? Why is it best to choose custom rabbit polyclonal antibodies over other common monoclonal or polyclonal antibodies such as mice or mice? To get around this, let's first look at antibodies and why they are so important for scientific and medical research.
Image Source: Google
Monoclonal antibodies have been traditionally bred in rodents such as mice because they are cheap and easier to use. The use of rabbits as the host species is increasingly becoming the preferred monoclonal antibody of choice for use in various research, diagnostic, and clinical applications because the rabbit's immune system is capable of producing more high-affinity antibodies in mice.
Rabbit monoclonal antibodies also respond better to immunogens and have higher epitope affinity and specificity.
With antibodies bred in rodents, the protein is more likely to be recognized as an antigen on its own, making it less immunogenic. In addition, rabbits tended to produce better antibodies against smaller peptides with smaller epitopes, which usually causes a poor